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SMAD2 antibody (SMAD, Mothers Against DPP Homolog 2)

Details for Product anti-SMAD2 Antibody No. ABIN121675, Supplier: Log in to see
Antigen
  • jv18
  • Xmad2
  • madh2
  • madr2
  • XSmad2
  • hmad-2
  • hsmad2
  • jv18-1
  • smad-2
  • fj43c06
  • wu:fj43c06
  • JV18
  • JV18-1
  • MADH2
  • MADR2
  • hMAD-2
  • hSMAD2
  • 7120426M23Rik
  • Madh2
  • Madr2
  • Smad-2
  • mMad2
Alternatives
anti-Human SMAD2 antibody for Western Blotting
Reactivity
Human
378
170
153
19
14
8
8
7
6
6
5
5
3
2
2
1
1
1
1
1
1
1
1
Host
Goat
329
46
13
Clonality
Polyclonal
Conjugate
This SMAD2 antibody is un-conjugated
13
11
7
7
7
7
6
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
308
175
77
75
42
29
28
27
15
9
6
6
5
3
1
Supplier
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Immunogen Synthetic peptide SEIWGLSTPNTID derived from MADH2 protein sequence
Purification Affinity chromatography
Alternative Name SMAD2 (SMAD2 Antibody Abstract)
Background This protein is a transcriptional modulator activated by TGF beta and may act as a tumour suppressor in colorectal carcinoma. It is found at high levels in skeletal muscle, heart and placenta. MADH2 exists in isoforms, a predominant long form and a short form missing the 30 amino acid exon 3. The short form shows DNA binding and higher transcriptional activation activity.Synonyms: JV18-1, MAD homolog 2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, SMAD 2, SMAD family member 2, SMAD-2
Research Area Signaling, Transcription Factors
Pathways Cell Division Cycle
Application Notes Western Blot: 1/200 - 1/500, this antibody detects bands of approximately 45 kDa and 55 kDa in HeLa cell lysates, a minimum incubation time of 1 hour is recommended with this antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer TRIS buffered saline pH 7.3 containing 0.02 % Sodium Azide and 0.5 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Eppert, Scherer, Ozcelik et al.: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).