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anti-NF-kB p65 / RELA antibody

Overview

Antigen: NF-kB p65 / RELA
Clonality: Polyclonal
Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Electrophoretic Mobility-Shift Assay (EMSA), Immunoprecipitation (IP), Western Blotting (WB)
Reactivity: Human, Mouse (Murine)
Host: Rabbit
Quantity: 0.1 ml (Product variants)
Price: Product not available in this region.
Order number: ABIN121887
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Additional Information  » anti-NF-kB p65 / RELA antibody

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Antigen NF-kB p65 / RELA
Swiss-Prot Q04206
Immunogen NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH
Reactivity Human, Mouse (Murine)
Clonality Polyclonal
Format Serum
Host Rabbit
Specificity This antibody is reactive with NFkB p65 subunit (Rel A). A control peptide that competes specifically with the antiserum is available ABIN126329.

Application Details  » anti-NF-kB p65 / RELA antibody

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Application Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Electrophoretic Mobility-Shift Assay (EMSA), Immunoprecipitation (IP), Western Blotting (WB)
» Click here to view 4 available protocols!
Application Notes ELISA. Immunoprecipitation. Western Blot: 1/2000 - 1/5000. Gel Super Shift Assays: 0.5-1ul per test, In a gel supershift assay it was found to be active against all p65 containing human and mouse NFkB complexes using 0.5ul to 1.0ul per assay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Buffer Containing 0.01% Sodium Azide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Restrictions For Research Use only