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Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (N-Term)

Details for Product anti-CASP4 Antibody No. ABIN121890, Supplier: Log in to see
Antigen
  • CASP4
  • ICE(rel)II
  • ICEREL-II
  • ICH-2
  • Mih1/TX
  • TX
  • Casp11
  • Caspl
  • ich-3
  • CASP1
  • CASP13
Alternatives
anti-Human Caspase 4 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
N-Term
41
26
14
10
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
162
72
30
20
2
1
1
Host
Rabbit
179
21
15
12
6
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
13
11
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
188
85
52
42
12
11
11
8
7
5
3
3
2
1
1
Supplier
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Immunogen A 16 amino acid peptide from human Caspase-4 amino terminus
Isotype IgG
Specificity This antibody recognises an epitope within the N-terminal region (NT) of Caspase-4, otherwise known as ICH-2. This antibody detects a band of approximately 34 kDa in Ramos cell lysates (predicted precursor MWT 31 kDa).
Purification Affinity Chromatography
Alternative Name Caspase-4 (CASP4 Antibody Abstract)
Background Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimers disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX
Gene ID 837
UniProt P49662
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot: 1.0 μg/mL. Immunohistochemistry on paraffin sections: 2.0 μg/mL, This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Spleen tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Katayama, Imaizumi, Manabe et al.: "Induction of neuronal death by ER stress in Alzheimer's disease." in: Journal of chemical neuroanatomy, Vol. 28, Issue 1-2, pp. 67-78, 2004 (PubMed).

Hitomi, Katayama, Eguchi et al.: "Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and Abeta-induced cell death." in: The Journal of cell biology, Vol. 165, Issue 3, pp. 347-56, 2004 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Kamens, Paskind, Hugunin et al.: "Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15250-6, 1995 (PubMed).