Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G) antibody

Antigen: Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G)

Synonyms: ARP9, CEM15, MDS019, FLJ12740, bK150C2.7, dJ494G10.1, APOBEC3G

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121896

Additional Information

Alternative name: APOBEC3G

UniProt: Q9HC16

Immunogen: A 15 amino acid peptide located near human APOBEC3G amino-terminus

Cross-Reactivity: Human

Isotype: IgG

Description: APOBEC3G is a 46kDa multi-isoform endogenous deoxycytidine deaminase, which plays a major role in the defence of T lymphocytes against retroviral infection. The incorporation of APOBEC3G into HIV-1 virion DNA, results in the deamination of dC to dU in minus-strand viral DNA during reverse transcription, thereby generating G to A hypermutations in the newly- synthesised viral DNA. The effect of APOBEC3G is counteracted by the HIV protein Vif, thought to induce the degradation of APOBEC3G through a ubiquitin-dependant proteasomal pathway. Alternate Names: APOBEC-3G, ARP9, CEM15, MDS019, ARCD.

Application Details

Application Notes: Western Blot: 5ug/ml, this antibody detects a band of approximately 46kDa in Human CaCO-2 cell lysates. Immunohistochemistry on paraffin sections: 1ug/ml. Recommended Positive Control: Spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Affinity Purified

Buffer: PBS containing 0.02% Sodium Azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Immunology, Chromatin Binding Proteins

Restrictions: For Research Use only

Publications

Zhang, Yang, Pomerantz et al.: "The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA." in: Nature, Vol. 424, Issue 6944, pp. 94-8, 2003 (PubMed).

Chiu, Soros, Kreisberg et al.: "Cellular APOBEC3G restricts HIV-1 infection in resting CD4+ T cells." in: Nature, Vol. 435, Issue 7038, pp. 108-14, 2005 (PubMed).

Andrade, Goncalves: "[Traumatic spinal cord injury: neurologic and functional recovery]" in: Acta médica portuguesa, Vol. 20, Issue 5, pp. 401-6, 2008 (PubMed).