Chemokine (C-C Motif) Receptor 3 (CCR3) antibody

Antigen: Chemokine (C-C Motif) Receptor 3 (CCR3)

Synonyms: CKR3, CD193, CMKBR3, CC-CKR-3, MGC102841

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN121929

Additional Information

Alternative name: CD193 / CCR3

Swiss-Prot: P51677

Immunogen: Synthetic peptide corresponding to a 14 amino acid sequence located near human CD193 carboxy-terminus

Description: CD193 acts as a receptor for several CC chemokines including monocyte chemoattractant proteins (MCP) -1, -2, -3 and -4, MIP-1 a , MIP-1ß, and RANTES. The eotaxin subgroup of CC chemokines act as specific agonists for CD193, and this interaction has become a focus area for studies into the infection of microglia by HIV (2) and also allergy research (3). Alternate Names: CCR3.

Specificity: This antibody recognises human CD193, otherwise known as CC chemokine receptor 3 (CCR3), a seven-helix transmembrane G-protein coupled receptor which, along with CCR5 and CXCR4, acts as a principal receptor for the fusion and entry of HIV into target cells. CD193 is expressed by eosinophils, basophils, mast cells, microglia, Th2 helper cells and some epithelial cells.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (10 ug/ml). Western blot (1 ug/ml, detects a band of approximately 55 kDa in human spleen cell lysates, predicted Mwt. 41 kDa). Use human spleen as positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: Phosphate buffered saline, containing 0.02 % Sodium Azide (NaN3)

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots, undiluted) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: one year from despatch.

Research Area: Chemokines, Receptors, CD Antigens

Restrictions: For Research Use only

Publications

Publications: Combadiere, Ahuja, Murphy: "Cloning and functional expression of a human eosinophil CC chemokine receptor." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16491-4, 1995 (PubMed).

He, Chen, Farzan et al.: "CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia." in: Nature, Vol. 385, Issue 6617, pp. 645-9, 1997 (PubMed).

Holse, Assing, Poulsen: "CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals." in: Clinical and molecular allergy : CMA, Vol. 4, pp. 6, 2006 (PubMed).