A Disintegrin-Like and Metalloprotease with Thrombospondin Type 1 Motif 5 (ADAMTS5) (C-Term) antibody

Antigen: A Disintegrin-Like and Metalloprotease with Thrombospondin Type 1 Motif 5 (ADAMTS5)

Synonyms: AAC2, PNAT, ADMP-1, ADAMTS-2, ADAMTS-4, KIAA0688, KIAA0366, NPI, PCINP, PCPNI, hPCPNI, ADAM-TS2, ADAMTS-3, TTP, VWFCP, C9orf8, vWF-CP, FLJ42993, MGC118899, MGC118900, DKFZp434C2322, ADMP-2, ADAMTS11, FLJ36738, ASMP-2, ADAMTS1, ADAM-TS5, AI481094, 9530092O11Rik, PWTSR, THSD2, CRISTIN1, FLJ14440, AW743315, 1810011L16Rik, 8430403M15Rik, E030027K14Rik, FLJ16042, AU015154, 4831407I23Rik, D230034E10Rik, ADAM-TS4, mKIAA0688, 3830423K05, Gm710, ADAMTS5, ADAMTS4, ADAMTS13, ADAMTS-11, METH2, ADAM-TS8, FLJ41712, ADAM-TS7, DKFZp434H204, ADAMTS7B

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121991

Additional Information

Alternative name: ADAMTS5 (C-term)

Swiss-Prot: Q9UNA0

Immunogen: Synthetic peptide - KLH conjugated.

Description: ADAMTS5, also known as aggrecanase-2, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan. The protein contains a signal sequence, a prodomain with both a potential cysteine switch and a furin cleavage site, a metalloproteinase domain with a conserved zinc-binding motif and a 'met turn,' a disintegrin- like domain, and a spacer region between a thrombospondin type 1 motif and thrombospondin submotif. At least four transcript variants have been reported. ADAMTS5 has been implicated in inflammatory joint diseases. ADAMTS5 expression has been documented in a range of normal human tissues. ESTs have been isolated from human tissue libraries, including normal breast, embryo and heart and cancerous placenta and uterus. Alternate Names: ADAMTS5, Aggrecanase-2, ADAM-TS5, Aggrecanase-2, ADMP2, ADMP-2, ADAMTS11 , A disintegrin and metalloproteinase with thrombospondin motifs 5.

Specificity: Reacts with human ADAMTS5 (family: Protease, subfamily: Metallopeptidase M12B). The immunizing peptide shows 94% identity / similarity to mouse and rat

Synonyms: AAC2, PNAT, ADMP-1, ADAMTS-2, ADAMTS-4, KIAA0688, KIAA0366, NPI, PCINP, PCPNI, hPCPNI, ADAM-TS2, ADAMTS-3, TTP, VWFCP, C9orf8, vWF-CP, FLJ42993, MGC118899, MGC118900, DKFZp434C2322, ADMP-2, ADAMTS11, FLJ36738, ASMP-2, ADAMTS1, ADAM-TS5, AI481094, 9530092O11Rik, PWTSR, THSD2, CRISTIN1, FLJ14440, AW743315, 1810011L16Rik, 8430403M15Rik, E030027K14Rik, FLJ16042, AU015154, 4831407I23Rik, D230034E10Rik, ADAM-TS4, mKIAA0688, 3830423K05, Gm710, ADAMTS5, ADAMTS4, ADAMTS13, ADAMTS-11, METH2, ADAM-TS8, FLJ41712, ADAM-TS7, DKFZp434H204, ADAMTS7B

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections (2-4 ug/ml). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 min, Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.7, containing 0.01% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or(in aliquots) at -20°C to -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Proteases, Extracellular Matrix

Restrictions: For Research Use only

Images

Immunohistochemical staining of cartilage using anti-ADAMTS5 antibody ABIN121991