TAS1R3
Reactivity: Human, Mouse
WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blot. Immunohistochemistry with formalin fixed paraffin embedded tissues: 17 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min, Remove from heat and let stand at room temperature in buffer for 20 min, Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date
12 months
Target
TAS1R3
(Taste Receptor, Type 1, Member 3 (TAS1R3))
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Background
The sense of taste provides animals with valuable information about the quality and nutritional value of food. There are four widely accepted categories of taste perception: sweet, bitter, salty and sour. A controversial fifth taste, known as umami or monosodium glutamate (MSG), has also been described. A family of G protein-coupled receptors are involved in taste perception and include T1R, which is involved in sweet and umami taste perception, and T2R, which is involved in bitter taste perception. The T1R family consists of three members: T1R1, T1R2 and T1R3. These proteins form heterodimers, which alter the selectivity of the subunits. The T1R2 and T1R3 heterodimer functions as a receptor for sweet taste, and recognizes several sweet-tasting molecules such as sucrose, saccharin, dulcin and acesulfame-K. The T1R1 and T1R3 heterodimer recognizes L-amino acids to perceive umami taste. Sweet taste transduction is carried out by two pathways. First, sucrose and other sugars activate Gas via the T1Rs, which activates adenylyl cyclase to generate cAMP. TAS1R3 is a Taste Receptor. TAS1R3 combines with TAS1R1 to function as an L-amino-acid receptor, and is only activated by these sweet amino acids.Synonyms: Sweet taste receptor T1R3, TR3, Taste receptor type 1 member 3