GPR65 antibody

Catalog No. ABIN122422

Product Details anti-GPR65 Antibody

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR65 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Reacts with T-Cell Death-Associated Gene 8 (family: GPCR, subfamily: Lysophospholipid/Lysosphingolipid)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 4-11 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GPR65

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Alternative Name: GPR65 / Psychosine Receptor (GPR65 Products)

Synonyms: tdag8 antibody, htdag8 antibody, TDAG8 antibody, hTDAG8 antibody, Dig1 antibody, Gpcr25 antibody, Tdag8 antibody, G protein-coupled receptor 65 antibody, G-protein coupled receptor 65 antibody, GPR65 antibody, gpr65 antibody, Gpr65 antibody

Background: GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the death of the myelin-synthesizing cells, oligodendrocytes, which is associated with Krabbe disease. Recent evidence suggests that GPR65 is one of the proton-sensing GPCRs coupling to adenylyl cyclase and psychosine and its related lysosphingolipids behave as if they were antagonists against protein-sensing receptors, including TDAG8, GPR4 and OGR1 GPR65 has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. ESTs have been isolated from human B-cell/lung/testis, brain/lung/testis, pancreas/spleen, lung/spleen, leukocyte, embryo, kidney, liver/spleen, thyroid, and vessel libraries.Synonyms: G-protein coupled receptor 65, T-cell death-associated protein 8, TDAG8

Gene ID: 8477

UniProt: Q8IYL9

Pathways: cAMP Metabolic Process