NR5A1 antibody (Internal Region)

Catalog No. ABIN122633

Product Details anti-NR5A1 Antibody

Target: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR5A1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Steroidogenic factor 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIM23 (47 %).

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: STF-1, SF-1, NR5A1, AD4BP, FTZF1, SF1, Nuclear receptor subfamily 5 group A member 1,Adrenal 4-binding protein, Steroid hormone receptor Ad4BP, Fushi tarazu factor homolog 1

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide - KLH conjugatedEpitope: internal domain of humanGenename: NR5A1Remarks: The immunizing peptide shows 88 % identity (100 % similarity) with mouse and88 % identity (100 % similarity) with rat.

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 2-18 μg/mLAntigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for NR5A1

Target: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Alternative Name: Steroidogenic Factor 1 (NR5A1 Products)

Synonyms: AD4BP antibody, ELP antibody, FTZ1 antibody, FTZF1 antibody, POF7 antibody, SF-1 antibody, SF1 antibody, SPGF8 antibody, SRXY3 antibody, Ad4BP antibody, ELP-3 antibody, Ftz-F1 antibody, Ftzf1 antibody, Ad4BP/SF-1 antibody, Ad4bp antibody, Sf-1 antibody, NR5A1 antibody, sf-1 antibody, elp antibody, sf1 antibody, ftz1 antibody, ad4bp antibody, ftzf1 antibody, SF-1/Ad4BP antibody, SI:zC167P9.2 antibody, ff1d antibody, nr5a2l antibody, nuclear receptor subfamily 5 group A member 1 antibody, nuclear receptor subfamily 5, group A, member 1 antibody, nuclear receptor subfamily 5 group A member 1 L homeolog antibody, nuclear receptor subfamily 6 group A member 1 antibody, nuclear receptor subfamily 5, group A, member 1b antibody, NR5A1 antibody, Nr5a1 antibody, nr5a1.L antibody, nr5a1 antibody, NR6A1 antibody, nr5a1b antibody

Background: Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.Synonyms: AD4BP, Adrenal 4-binding protein, FTZF1, Fushi tarazu factor homolog 1, NR5A1, Nuclear receptor subfamily 5 group A member 1, SF-1, SF1, STF-1, Steroid hormone receptor Ad4BP

Gene ID: 2516

UniProt: Q13285

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Maintenance of Protein Location