Crystallin, alpha A (CRYAA) antibody

Antigen: Crystallin, alpha A (CRYAA)

Synonyms: CRYA1, HSPB4, Crya1, lop18, Acry-1, Crya-1, DAcry-1, AI323437, cryaa-A, CRYAA, fc06h04, MGC92036, zgc:92036, wu:fc06h04, alpha-a-crystallin

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN122707

Additional Information

Alternative name: Alpha-crystallin A chain

Swiss-Prot: P02489

Immunogen: Purified alpha-A crystallin from bovine lens

Description: Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted. Alternate Names: Alpha-crystallin A chain, Heat shock protein beta-4, HSPB4, CRYA1, Heat shock protein beta 4, Alpha-A crystallin, Zonular Central Nuclear Cataract, Crystallin Alpha 1.

Specificity: ABIN122707 detects alpha-A crystallin. It does not detect the unphosphorylated form of the protein.

Application Details

Application Notes: Western Blot: 0.1 ug/ml, detects an ~20 kDa protein representing alpha-A crystallin from bovine lens samples. Immunohistochemistry on Paraffin embedded sections: 10 ug/ml, results in staining of epithelial reticular cells in rat thymus samples. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 30 mg/ml BSA and 0.05% sodium azide as preservative

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Neurology, Enzymes

Restrictions: For Research Use only

Images

Figure 1 shows a Western blot of alpha-A crystallin from bovine lens extract