Adenosine A1 Receptor (ADORA1) antibody

Antigen: Adenosine A1 Receptor (ADORA1)

Synonyms: RDC7, Ri, A1R, A1AR, AA1R, A1-AR, AI848715, BB176431, ADORA1, adora1-A, a1R, LOC606743, Adora1, DKFZp469D1021, LOC100222054, LOC100328615

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Rat (Rattus)

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN122729

Additional Information

Alternative name: Adenosine receptor A1

Swiss-Prot: P30542

Immunogen: ABIN122729 immunizing peptide corresponds to amino acid residues 309-326 from rat A1AR: C(309) Q P K P P I D E D L P E E K A E D(326)

Description: Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Specificity: This antibody detects adenosine receptor A1 (A1AR) in human, rat and bovine tissues. It does not detect other AR subtypes.

Application Details

Application Notes: Western blot: 1/500, detects an ~37 kDa protein in rat brain representing A1AR. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cell Cycle, DNA/RNA, Receptors

Restrictions: For Research Use only