Adenosine A2a Receptor (ADORA2A) antibody

Antigen: Adenosine A2a Receptor (ADORA2A)

Synonyms: ADORA2A, adora2a.1, zgc:194773, LOC606744, LOC100034039, Adora2a, RDC8, hA2aR, ADORA2, A2aR, A2AAR, AA2AR, MGC118414, A2ar, ADENO, Adora2l1, MGC93190

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Human, Rat (Rattus)

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN122730

Additional Information

Alternative name: Adenosine receptor A2a

Swiss-Prot: P29274

Immunogen: Synthetic Peptide: E(373) S H G D M G L P D V E L L S H E L K(391) corresponding to amino acids 373-391 of canine A2aAR

Description: Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A2aAR is a glycoprotein that can activate adenylyl cyclase via Gs interactions. Stimulation of A2aAR and the resultant accumulation of cAMP inhibits platelet aggregation and in certain vascular beds is associated with vasodilation and drop in blood pressure. A2aAR is reported to interact with D2 dopamine receptor sites in the brain where they co-express (striatum, olfactory tubercle and nucleus accumbens). Other tissues including heart, kidney, and lung also express A2aAR. Alternate Names: Adenosine Receptor A2a, ADORA2A, ADORA2, FocusOn65 , Adenosine receptor subtype A2a, RDC8, hA2aR.

Specificity: This antibody detects adenosine receptor A2a (A2aAR) in human, rat and canine tissues. This antibody does not detect other AR subtypes.

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: 1/100, staining of A2aAR in human hippocampus with ABIN122730 yields a pattern consistent with plasma membrane staining. Western Blot: 1/1000, detects an ~45 kDa protein from canine striatum representing A2aAR. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.69 mg/ml

Buffer: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cell Cycle, DNA/RNA, Receptors

Restrictions: For Research Use only