Adrenergic, alpha-1A-, Receptor (ADRA1A) (pan alpha 1) antibody

Antigen: Adrenergic, alpha-1A-, Receptor (ADRA1A)

Synonyms: DAR, ADRA1, ADRA1A, ADRA1R, ALPHA1, dJ779E11.2, ADRA1C, ADRA1L1, ALPHA1AAR, [a]1a, Adra1c, MGC130524, fc18h12, wu:fc18h12, ALPHA-1A, Adra1a, alpha-1A

Epitope: pan alpha 1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN122735

Additional Information

Alternative name: Alpha-1A adrenergic receptor (pan alpha 1)

Swiss-Prot: P35348

Immunogen: Synthetic Peptide corresponding to amino acid residues 339-349 in the 3rd intracellular loop of human A1AR. This sequence is 100% conserved in all A1AR subtypes examined. AA Sequence:K(339) F S R E K K A A K T(349)

Description: Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose Western blot under reducing conditions (3 µg/lane Apolipoprotein E), this antibody gave a major band at ~30 kDa when used at 1/500-1/1000., heat shock protein 70 (HSP70), proto- oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.

Specificity: This antibody detects alpha-1 adrenergic receptor (A1AR) from human, rat, mouse, zebra fish (Danio rerio) and Drosophila samples.

Application Details

Application Notes: Immunofluorescence (1/1000, results in staining of the plasma membrane in mouse kidney distal tubule cells). Western Blot (1/400, detects an ~60 kDa protein representing the A1AR from mouse kidney membrane preparations, mouse liver, HeLa cell lysates, zebra fish and drosphila samples). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: ABIN122735 immunizing peptide (ABIN126358) is available for use in neutralization and control experiments.

Concentration: 0.5 mg/ml

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Receptors

Restrictions: For Research Use only

Images

Figure 1 shows immunolocalization of A1AR in mouse distal convoluted tuble using ABIN122735.