Period Homolog 1 (Drosophila) (PER1) antibody

Antigen: Period Homolog 1 (Drosophila) (PER1)

Synonyms: PER, hPER, RIGUI, MGC88021, per1, zC237L4.4, si:ch211-237l4.4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN122822

Additional Information

Alternative name: PER1

Swiss-Prot: O15534

Immunogen: ABIN122822 immunizing peptide corresponds to amino acid residues 39-51 from mouse PER1: P(39) S L A D D T D A N S N G(51) C

Description: Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex. Alternate Names: Per1.

Specificity: This antibody detects PER1 from mouse tissues as well as recombinant mouse PER1. ABIN122822 immunizing peptide (ABIN126418) is available for use in Neutralization and Control experiments

Application Details

Application Notes: Western Blot: 1/200, detects overexpressed recombinant mouse PER1 protein. Immunofluorescence staining of PER1 in mouse testes with ABIN122822 results in cytoplasmic staining. Immunohistochemistry on frozen and Paraffin embedded sections: 1/500. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only