Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) antibody

Antigen: Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4)

Synonyms: CaMK-GR, MGC36771, CAMK4, CaMKIV, AI666733, CaMKIV/Gr, D18Bwg0362e, A430110E23Rik, Ccdpk, RATCCDPK, camk4-A, wu:fj42a03, zgc:110275

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Human, Mouse (Murine), Primate, Rat (Rattus), Xenopus laevis

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN122826

Additional Information

Alternative name: CAMK4

Swiss-Prot: Q16566

Immunogen: Synthetic Peptide: V(127) E K G Y Y S E R D A A D A V K Q(143) C corresponding to amino acid residues 127-143 from mouse CaMKIV.

Description: CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium- dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids. Alternate Names: CAMK4, CAMK-4, CAMKIV, CAM kinase-GR, FocusOn84, Calcium/calmodulin-dependent protein kinase type IV.

Specificity: This antibody detects CaM kinase IV (CaMKIV) from arabidopsis, canine, human, mouse, primate, rat tissues and xenopus oocyte samples. This sequence is 94% conserved in rat and human CaMKIV. This peptide (ABIN126422) is available for use in Neutralization and Control experiments

Application Details

Application Notes: Western Blot: 0.5 ug/ml, detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. Immunohistochemistry on frozen sections: 1 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Kinases/Phosphatases

Restrictions: For Research Use only

Images

Figure 1 shows a Western blot of CaMKIV in rat brain extract using ABIN122826.