S-arrestin (SAG) antibody

Antigen: S-arrestin (SAG)

Synonyms: RP47, S-AG, DKFZp686D1084, DKFZp686I1383

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN122855

Additional Information

Alternative name: S-arrestin / SAG

Swiss-Prot: P15887

Immunogen: Synthetic Peptide: C E(347) V A T E V P F R L M H P Q P E D(363) corresponding to amino acid residues 2-18 from rat visual arrestin.

Description: Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin.

Specificity: This antibody detects recombinant bovine visual arrestin. This peptide (ABIN126447) is available for use in Neutralization and Control experiments

Application Details

Application Notes: Western Blot: 3 ug/ml, detects an ~57 kDa protein representing recombinant bovine visual arrestin. This antibody also detects a lower molecular weight protein which could correspond to degradation product. Immunofluorescence: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only

Images

Figure 1. Western blot on recombinant bovine visual arrestin using ABIN122855.