Cannabinoid Receptor 1 (CNR1) antibody

Antigen: Cannabinoid Receptor 1 (CNR1)

Synonyms: CB1, CNR, CB-R, CB1A, CB1R, CANN6, CB1K5, SKR6R, CNR1, LOC540382

Clonality: Polyclonal

Host: Rabbit

Reactivity: Amphibian, Chicken, Fish, Human, Mouse (Murine), Primate, Rat (Rattus)

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN122865

Additional Information

Alternative name: Cannabinoid receptor 1

Swiss-Prot: P21554

Immunogen: A fusion protein containing the first 77 amino acid residues from rat CB1

Description: Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9- tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina. Alternate Names: CB1, CB-R, CANN6, CNR, FocusOn74.

Specificity: This antibody detects Cannabinoid Receptor 1 (CB1).

Application Details

Application Notes: FACS. Immunohistochemistry on Paraffin Sections. Immunocytochemistry: 1/1,000, results in intense, punctate staining along processes, with little staining over cell bodies in living or fixed cultured hippocampal neurons. Western Blot: detects an ~60 kDa protein representing CB1 and less intense bands of ~23 kDa, ~72 kDa, and ~180 kDa from rat brain homogenate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 3.3 mg/ml

Buffer: PBS containing 50% Glycerol, 1 mg/ml BSA as stabilizer and 0.05% Sodium Azide as preservative

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: One year from despatch.

Research Area: Receptors

Restrictions: For Research Use only

Images

Figure 1 shows immunolocalization of cannibinoid receptor 1 in rat hippocampus.