V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (EQKLISEEDL) antibody

Antigen: V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyms: MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746

Epitope: EQKLISEEDL

Clonality: Polyclonal

Host: Rabbit

Application: Immunofluorescence (IF)

Catalog no.: ABIN122985

Additional Information

Characteristics: Synonyms: myc tag, myc-tag, c-myc tag

Alternative name: C-Myc Epitope Tag (EQKLISEEDL)

Gene ID: 4609

Swiss-Prot: P01106

Immunogen: Synthetic peptide corresponding to amino acid residues 410-419 of human cMyc protein. AA Sequence: C E(410) Q K L I S E E D L(419)

Description: Epitope tagging is a powerful and versatile strategy for detecting and purifying proteinsexpressed by cloned genes. To utilize this feature, protein expression vectors are typicallyengineered with a nucleotide sequence that encodes the peptide epitope tag. The gene ofinterest is cloned in-frame relative to the tag and, upon expression, the protein of interestis synthesized as a fusion protein with the peptide tag. Fusion protein detection and/orpurification is mediated by highly specific antibodies to the engineered peptide, thuseliminating the need for antibodies to proteins from each newly cloned gene. Commonlyused epitope tags include glutathione-S-transferase (GST), 6-histidine (6X-His), greenfluorescent protein (GFP), c-myc and others. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferationand differentiation. In addition, deregulated expression of c-Myc induces apoptosis indifferent cell types, with c-myc requiring p53 for apoptosis in many cell types. This factindicates heterogeneous mechanisms for c-myc-induced apoptosis.

Specificity: The antibody detects the presence of expressed cMyc-tagged proteins in transfectedmammalian cells. Add. Information: ABIN122985 immunizing peptide (ABIN126538) is available for use in neutralization and controlexperiments.

Application Details

Application Notes: Immunofluorescence (1/100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1.0 mg/ml BSA and 0.05 % sodium azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Transcription Factors, Tags/Labels

Restrictions: For Research Use only

Images

Figure 1 shows immunolocalization of cMyc in transfected mammalian cells using ABIN122985.