Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A) antibody

Antigen: Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A)

Synonyms: APH-1, APH-1A, CGI-78, 6530402N02Rik, RGD1311546, psfl, APH1A, wu:fc41h05, wu:fi53a04, MGC128098, aph-1a, cgi-78, MGC97845, aph1a, MGC7221, MGC36389, APH, OPH, ACPH, D3S48E, D3F15S2, DNF15S2, MGC2178, AAP, AARE

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN123019

Additional Information

Alternative name: APH1A

UniProt: Q96BI3

Immunogen: The immunogen is a synthetic peptide, KLH conjugated, corresponding to residues 245-265 of the C-Terminal domain of the long isoform of APH-1a. AA Sequence:C(245) R R Q E D S R V M V Y S A L R I P P E D (265)

Cross-Reactivity: Human, Mouse (Murine)

Description: Familial Alzheimer's disease is often characterized by an early ( < 60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. APH-1 has been found to be a member of the presenilin (PS) complex, and likely contributes to the gamma-secretase activity involved with the onset of Alzheimer's. Recent evidence has shown that APH-1 also plays a role in the maturation of nicastrin/presenilin complexes.

Specificity: The antibody detects APH-1.

Synonyms: APH-1, APH-1A, CGI-78, 6530402N02Rik, RGD1311546, psfl, APH1A, wu:fc41h05, wu:fi53a04, MGC128098, aph-1a, cgi-78, MGC97845, aph1a, MGC7221, MGC36389, APH, OPH, ACPH, D3S48E, D3F15S2, DNF15S2, MGC2178, AAP, AARE

Application Details

Application Notes: Western Blot (1/1000): detects a ~18 kDa protein representing APH-1 in mouse brain and kidney extracts. Immunofluorescence (1/100). Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This polyclonal antibody has been referred to as O2C2.

Concentration: 0.69 mg/ml

Purification: Aff - Purified

Buffer: PBS containing 1 mg/ml BSA and 0.005% sodium azide and glycerol

Storage: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Figure 1 shows a Western blot of APH-1 in mouse brain and kidney extracts using ABIN123019.

Figure 2 shows immunofluorescence staining of APH-1 in human KNS-42 glioma cells using ABIN123019.

Publications

Gu, Chen, Sanjo et al.: "APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7374-80, 2003 (PubMed).