Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) antibody

Antigen: Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1)

Synonyms: htpG

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN123041

Additional Information

Alternative name: HSP90AA1 / HSP90 alpha

Swiss-Prot: P07900

Immunogen: Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mosue HSP86

Description: Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP90 exists as two forms, HSP84 and HSP86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP90, HSP89 beta and HSP89 alpha. In an unstressed mouse fibroblast, the basal level of HSP84 is found to be double that of HSP86. However, after heat shock, HSP86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP86, but not HSP84, decreases. HSP84 and HSP86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.

Specificity: The antibody detects heat shock protein 86 (HSP86), it does not react with HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence

Application Details

Application Notes: Immunofluorescence (10 - 15 ug/ml): Immunofluorescence staining of HSP86 in HeLa cells yields a pattern consistent with cytoplasmic staining. Immunohistochemistry (paraffin) : 5 ug/ml. Immunoprecipitation : Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells. Furthermore, the antibody is capable of precipitating HSP86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor. Western blot (0.5 ug/ml): By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Figure 1 shows a Western blot of mouse HSP84 and HSP86 using ABIN122993 and ABIN123041 respectively.