Serotonin Receptor 1B (HTR1B) antibody

Antigen: Serotonin Receptor 1B (HTR1B)

Synonyms: S12, 5-HT1B, HTR1D2, HTR1DB, 5-HT1DB, 5-HT-dro2B, 5-HT1BDro, 5-HT[[1BDro]], 5-HT[[dro2B]], 5HT-R2B, 5HT-dro2B, 5HT-dro[[2B]], 5htdro2b, DRO2B, Dm5HTdro2B, d5-HT1B, DmelCG15113, CG15113, HTR1B, 5-HT1Db, 5-HT1-LIKE

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123273

Additional Information

UniProt: P28564

Immunogen: Mixture of synthetic peptides corresponding to amino acids 8-26 and 263-278 of rat 5-HT1BR (6).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1). Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase (2). Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum (3,4). The in situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei (5).

Specificity: This polyclonal antibody detects 5-HT1BR in brain cell lysates.

Synonyms: S12, 5-HT1B, HTR1D2, HTR1DB, 5-HT1DB, 5-HT-dro2B, 5-HT1BDro, 5-HT[[1BDro]], 5-HT[[dro2B]], 5HT-R2B, 5HT-dro2B, 5HT-dro[[2B]], 5htdro2b, DRO2B, Dm5HTdro2B, d5-HT1B, DmelCG15113, CG15113, HTR1B, 5-HT1Db, 5-HT1-LIKE

Application Details

Application Notes: Western blotting (use at 1 ug/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS pH 7.2 with 0.02% sodium azide.

Storage: Store the antibody at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 5-HT1BR in human brain lysate with anti-5-HT1BR pcAb ABIN123273. A protein band of approximate molecular weight of 40-41kDa was detected

Publications

Pazos, Palacios: "Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors." in: Brain research, Vol. 346, Issue 2, pp. 205-30, 1985 (PubMed).

Pazos, Cortes, Palacios: "Quantitative autoradiographic mapping of serotonin receptors in the rat brain. II. Serotonin-2 receptors." in: Brain research, Vol. 346, Issue 2, pp. 231-49, 1985 (PubMed).

Bruinvels, Landwehrmeyer, Gustafson et al.: "Localization of 5-HT1B, 5-HT1D alpha, 5-HT1E and 5-HT1F receptor messenger RNA in rodent and primate brain." in: Neuropharmacology, Vol. 33, Issue 3-4, pp. 367-86, 1994 (PubMed).