Serotonin Receptor 1D (HTR1D) antibody

Antigen: Serotonin Receptor 1D (HTR1D)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN123274

Additional Information

UniProt: P28221

Immunogen: Mixture of synthetic peptides corresponding to amino acids 1-18 and 251-267 of rat 5-HT1DR (Genbank accession no. NP_036984) (7).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1,2). The 5-HT1D (formerly known as 5- HT1Da) receptor has 63% overall structural homology with the 5-HT1B receptor and a 77% amino acid sequence homology in the seven transmembrane domains (3). In situ hybridization studies have detected highest level of mRNA in primary olfactory cortex, accumbens nucleus, caudate putamen, lateral mammilary nucleus and medial vestibular nucleus (4). The presence of relatively high levels of 5-HT1D mRNA in the locus coeruleus suggest that, when expressed on nerve terminals, this receptor could modulate the release of catecholamines. The 5-HT1D receptor may function as an autoreceptor. Like 5-HT1B receptors, 5-HT1D receptors also appear to act as heteroreceptors. Acting on the 5-HT1D receptors, 5-HT appears to inhibit release of glutamate from rat cerebellar synaptosomes and acetylcholine from guinea pig hippocampal synaptosomes (5). It has been suggested that neurogenic inflammation and nociceptive activity within trigemino- vascular afferents. Endothelium-dependent relaxation in the pig coronary artery has been claimed to be mediated by 5-HT1D receptors (6). Like other members of the 5- HT1 family, 5-HT1D receptors are negatively coupled to adenylate cyclase.

Specificity: This polyclonal antibody can be used for detection of 5-HT1DR in brain.

Application Details

Application Notes: Western blotting (2 ug/ml). A 40 kDa band is detected. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 5-HT1DR in brain lysate with anti-5-HT1DR pcAb ABIN123274. A protein band of approximate molecular weight of 40 kDa was detected

Publications

Weinshank, Zgombick, Macchi et al.: "Human serotonin 1D receptor is encoded by a subfamily of two distinct genes: 5-HT1D alpha and 5-HT1D beta." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3630-4, 1992 (PubMed).

Miller, Teitler: "Quantitative autoradiography of 5-CT-sensitive (5-HT1D) and 5-CT-insensitive (5-HT1E) serotonin receptors in human brain." in: Neuroscience letters, Vol. 136, Issue 2, pp. 223-6, 1992 (PubMed).

Lauder: "Neurotransmitters as growth regulatory signals: role of receptors and second messengers." in: Trends in neurosciences, Vol. 16, Issue 6, pp. 233-40, 1993 (PubMed).

Gerhardt, van Heerikhuizen: "Functional characteristics of heterologously expressed 5-HT receptors." in: European journal of pharmacology, Vol. 334, Issue 1, pp. 1-23, 1998 (PubMed).