Serotonin Receptor 3A (HTR3A) antibody

Antigen: Serotonin Receptor 3A (HTR3A)

Synonyms: HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R, 5-HT3, 5HT3, Htr3, MGC52789, HTR3A, Htr3a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN123275

Additional Information

UniProt: P46098

Immunogen: Mixture of synthetic peptides corresponding to amino acids 21-36 and 421-436 of rat 5-HT3 receptor

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1). The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems (2,3). Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids (4). The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse (5) and rat (6) receptors, respectively.

Application Details

Application Notes: Western blot (1 ug/ml). Recommended positive control: human brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 5-HT3R in human brain tissue lysate with anti-5-HT3R pcAb ABIN123275. A protein band of approximate molecular weight of 47-50 kDa

Publications

Maricq, Peterson, Brake et al.: "Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel." in: Science (New York, N.Y.), Vol. 254, Issue 5030, pp. 432-7, 1991 (PubMed).

Derkach, Surprenant, North: "5-HT3 receptors are membrane ion channels." in: Nature, Vol. 339, Issue 6227, pp. 706-9, 1989 (PubMed).

Morales, Battenberg, de Lecea et al.: "Cellular and subcellular immunolocalization of the type 3 serotonin receptor in the rat central nervous system." in: Brain research. Molecular brain research, Vol. 36, Issue 2, pp. 251-60, 1996 (PubMed).