V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)

Synonyms: PKBB, PRKBB, PKBBETA, RAC-BETA, AKT2, cb945, prkbb, v-akt2, pkbbeta, rac-beta

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123279

Additional Information

Alternative name: AKT2 / PKB beta

UniProt: P31751

Immunogen: Human Akt2. AA Sequence:319-331

Cross-Reactivity: Human

Description: Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

Specificity: This antibody detects Akt2. This sequence is 100 % homologous in Akt3

Application Details

Application Notes: Western blotting (1-2 ug/ml). Use HeLa as positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide as preservative.

Storage: Store the antibody at 2 - 8 °C for one month or at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of Akt2/3 using ABIN123279 at 1 µg/ml against 15 µg of HeLa lysate.

Publications

Cheng, Godwin, Bellacosa et al.: "AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 9267-71, 1992 (PubMed).

Cheng, Ruggeri, Klein et al.: "Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3636-41, 1996 (PubMed).

Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).