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V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody

Details for Product No. ABIN123280
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Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(162), (67), (54), (37), (34), (20), (17), (1), (1), (1)
Host
Rabbit
(110), (42), (9), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(120), (88), (32), (28), (26), (10), (6), (5), (4), (4), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN123280
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Immunogen Swiss-Prot ID: Q9Y243
Purification Protein G Chromatography
Alternative Name AKT3 / PKB gamma
Background Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy- terminal region of the protein. Akt/PKB phosphorylation is critical for its activity.
UniProt Q9Y243
Research Area Signaling
Application Notes Western blot: 1-2 ug/ml, A 50 kDa band is observed. Recommended Positive Control : HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% sodium azide, 0.1 mg/ml BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: 6 month from despatch.
Expiry Date 6 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody Western blot analysis for AKT3 using ABIN123280 at 2 µg/ml dilution against 15 µg/lane of HeLa cell lysate.
General Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).

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