V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123280

Additional Information

Alternative name: AKT3 / PKB gamma

UniProt: Q9Y243

Immunogen: Swiss-Prot ID: Q9Y243

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy- terminal region of the protein. Akt/PKB phosphorylation is critical for its activity.

Application Details

Application Notes: Western blot: 1-2 ug/ml, A 50 kDa band is observed. Recommended Positive Control : HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide, 0.1 mg/ml BSA

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: 6 month from despatch.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis for AKT3 using ABIN123280 at 2 µg/ml dilution against 15 µg/lane of HeLa cell lysate.

Publications

Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).