Bacillus Anthracis Lethal Factor (LF) antibody

Antigen: Bacillus Anthracis Lethal Factor (LF)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Bacillus anthracis

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123282

Additional Information

Alternative name: Bacillus anthracis lethal factor

UniProt: P15917

Immunogen: Synthetic peptides corresponding to amino acids 47-65 of the Anthrax LF protein (~90 kD)

Cross-Reactivity: Human

Description: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family (2).

Application Details

Application Notes: Western blot: 1/1000. Recommended positive control: recombinant protein. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Serum

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Bacteria, Gram positive bacteria, Toxins, Virology

Restrictions: For Research Use only

Images

Western blot analysis of Anthrax LF in recombinant protein using ABIN123282 (lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

Publications

Collier, Young: "Anthrax toxin." in: Annual review of cell and developmental biology, Vol. 19, pp. 45-70, 2003 (PubMed).