Ankyrin Repeat and SOCS Box-Containing 3 (ASB3) antibody

Antigen: Ankyrin Repeat and SOCS Box-Containing 3 (ASB3)

Synonyms: ASB-3, MGC996, FLJ10123, FLJ10421, MGC12531, MGC132002, 2400011J03Rik, fi36b12, zgc:56163, wu:fi36b12, ASB3, MGC142427, DKFZp459D0910

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123293

Additional Information

Alternative name: ASB3

UniProt: Q9Y575

Immunogen: Synthetic peptides corresponding to amino acids 63-75 and 166-180 of human Asb-3

Cross-Reactivity: Human

Description: The ankyrin repeat and SOCS box-containing (Asb) protein family is comprised of members which each contain SOCS boxes but differ from other families of protein in the motifs they contain upstream of the SOCS box(1). SOCS boxes are carboxy-terminal regions of homology found in the suppressor of cytokine signaling family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases.

Application Details

Application Notes: Western blot: 1/500 - 1/1000. Recommended positive control: human spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Serum

Buffer: PBS containing 0.02 % sodium azide and 0.1 mg/ml BSA

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of Asb-3 in 15 ugs of human spleen cell lysate using ABIN123293 at 1:1000 dilution.

Western blot analysis of Asb-3 in 15 ugs of human spleen cell lysate using ABIN123293 at 1:1000 dilution.

Publications

Krebs, Hilton: "SOCS proteins: negative regulators of cytokine signaling." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 5, pp. 378-87, 2001 (PubMed).

Wang, Campbell: "Cytokine signaling in the brain: putting a SOCS in it?" in: Journal of neuroscience research, Vol. 67, Issue 4, pp. 423-7, 2002 (PubMed).