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Antibody
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anti-Activating Transcription Factor 2 ATF2
Activating Transcription Factor 2 (ATF2) antibody
| Antigen |
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| Synonyms |
HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (24), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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| Catalog no. |
ABIN123294 |
| Quantity |
0.1 mg (0.5 mg/ml) (Variants) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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ATF2
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Swiss-Prot
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P15336
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Immunogen
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Swiss-Prot ID: P15336
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Description
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ATF-2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF-2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites (1-3). Deletion analysis has indicated that the N-terminal region of ATF-2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.
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Specificity
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This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acids 13-27.
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Application Notes
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Western blot: 2 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Buffer
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PBS containing 0.02% sodium azide.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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Immunology, Innate Immunity, Transcription Factors
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Restrictions
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For Research Use only
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Publications
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Hai, Curran: "Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 9, pp. 3720-4, 1991 (PubMed).
Benbrook, Jones: "Heterodimer formation between CREB and JUN proteins." in: Oncogene, Vol. 5, Issue 3, pp. 295-302, 1990 (PubMed).
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Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
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Hosts
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Rabbit (142), Mouse (16), Goat (4), Sheep (3)
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Reactivities
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Human (162), Mouse (Murine) (112), Rat (Rattus) (99), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1)
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Applications
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Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (24), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr69/51 (6), Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pSer62/44 (5), pThr69 or 51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer112/94 (4), pThr71/53 (4), pThr73/55 (3), S44 (2), S94 (2), PSer340/322 (1), S322 (1), pSer44 (1), pSer472 (1), pSer94 (1), pThr51 (1), pThr69/51 + pThr71/53 (1), pThr71 (1), pThr79/51 (1)
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Alternatives 
