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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) antibody
|Synonyms||DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, RGD1561519|
Alternatives Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml) (Variants)|
|Price||319.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||CD257 / BAFF|
|Immunogen||C-terminal peptide corresponding to amino acids 254-269 of human BAFF|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Simultaneously four different laboratories identified a new member of the tumor necrosis factor (TNF) family. This has been named as TALL-1 (6), THANK (TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase (5), BAFF (for B cell activating factor belonging to the TNF family) (4) and Blys (B lymphocyte stimulator) (3). Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases. Secreted BLyS/BAFF/THANK acts as a potent B cell growth factor. This protein is expressed abundantly in peripheral blood lymphocytes, specifically in monocytes and macrophages. Its regulation is upregulated by interferon gamma. Overexpression of BLyS/BAFF in transgenic mice lead to increased numbers of mature B and effector T cells. These mice also develop autoimmune -like symptoms, such as, high levels of rheumatoid factors, anti-DNA autoantibodies, etc. (2). Recently, two receptors for BLyS/BAFF have been identified and termed as BCMA and TACI (1). Alternate Names: BLyS, Thank, TALL-1.|
|Specificity||This antibody is specific for BAFF.|
|Application Notes||Western blot: 0.5 - 2.0 ug/ml. Recommended positive control: mouse testis, thymus, or spleen, or HL60. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.2 % gelatin, 0.05 % sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of BAFF/BLyS in mouse and human cell lysates. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human).|
Shu, Hu, Johnson: "TALL-1 is a novel member of the TNF family that is down-regulated by mitogens." in: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 680-3, 1999 (PubMed).
Mukhopadhyay, Ni, Zhai et al.: "Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 15978-81, 1999 (PubMed).
Schneider, MacKay, Steiner et al.: "BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1747-56, 1999 (PubMed).
Moore, Belvedere, Orr et al.: "BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 260-3, 1999 (PubMed).
Mackay, Woodcock, Lawton et al.: "Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations." in: The Journal of experimental medicine, Vol. 190, Issue 11, pp. 1697-710, 2000 (PubMed).
|Hosts||Rabbit (57), Mouse (30), Rat (23), Goat (9)|
|Reactivities||Human (105), Mouse (Murine) (32), Rat (Rattus) (29)|
|Applications||Western Blotting (WB) (70), ELISA (56), Flow Cytometry (FACS) (28), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Functional Studies (Func) (7), Immunocytochemistry (ICC) (7), Immunoprecipitation (IP) (6), Blocking Antibody (Inhibition) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Blocking Antibody (Inhibition) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (10), FITC (6), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (9), AA 83-285 (7), AA 254-269 (6), AA 134-285 (3), N-Term (2), Extracellular Domain (1)|