DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody

Details for Product No. ABIN123301
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Antigen
Epitope
AA 205-222
(16), (10), (9), (9), (7), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Guinea Pig, Mouse (Murine)
(79), (69), (45), (12), (11), (2)
Host
Rabbit
(110), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(97), (38), (32), (23), (19), (14), (10), (9), (2), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Availability Discontinued
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Catalog No. ABIN123301
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Immunogen Peptide corresponding to amino acids 205 to 222 of mouse CAD (1)
Purification Protein G chromatography
Alternative Name DFFB / CAD
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei1 (4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
UniProt O54788
Application Notes IHC (paraffin): 1:1000 (see Watanabe et al. for details). Western blot: 1/500 - 1/1000. Recommended positive control: mouse lung. A 40 kDa band should be observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody Western blot analysis of 50 ug of murine lung tissue lysate with anti-CAD (I17) at 1:500 dilution.
General Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Halenbeck, MacDonald, Roulston et al.: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

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