Caspase 12 (CASP12) antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN123303 |
| Quantity |
0.1 mg (0.5 mg/ml) (Variants) |
| Price |
594.00 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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Caspase-12
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UniProt
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Q6UXS9
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Immunogen
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Synthetic peptide corresponding to amino acids 2-17 of murine Caspase-12
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Cross-Reactivity
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Human, Mouse (Murine), Rat (Rattus)
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Description
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Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. A newly discovered member of this family Caspase-12, is localized to the endoplasmic reticulum (ER) but not to the cytoplasm or mitochondrion. In the ER it is associated with proteins that are involved in Alzheimer disease.
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Application Notes
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Western blotting: 0.5-1 ug/ml. Recommended positive control: mouse spleen. Immunohistochemistry on paraffin embedded tissues: 10 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Purification
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Purified
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Buffer
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PBS, pH 7.2, with 0.02% sodium azide
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Storage
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Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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Apoptosis/Necrosis
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Restrictions
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For Research Use only
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Nakagawa, Zhu, Morishima et al.: "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta." in: Nature, Vol. 403, Issue 6765, pp. 98-103, 2000 (PubMed).
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Alternatives for antigen "Caspase 12 (CASP12)", type "Antibodies"
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Hosts
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Rabbit (72), Rat (3), Goat (2)
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Reactivities
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Human (49), Mouse (Murine) (49), Rat (Rattus) (44)
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Applications
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Western Blotting (WB) (47), Immunofluorescence (IF) (35), ELISA (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Immunohistochemistry (IHC) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (4), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Epitopes
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Middle Region (37), N-Term (6), AA 100-116 (4), AA 2-17 (2), Center (2), Large Isoform (2), Small Isoform (2), AA 232-244 (1), Internal Region (1), Internal Region,AA 172-201 (1), Internal Region,AA 232-244 (1)
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