Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) antibody

Antigen: Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10)

Synonyms: MCH4, ALPS2, FLICE2, xCaspase-10, mch4, alps2, flice2, MGC107844, caspace-10, caspase-10, xCaspace-10, LOC100101580

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123304

Additional Information

Alternative name: Caspase-10

UniProt: Q92851

Immunogen: Peptide corresponding to amino acids 505 to 521 at the C-terminus of human FLICE2 (1)

Cross-Reactivity: Human

Description: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4 (1,2) and renamed as Caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis (1). Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death (3). Caspase-10 is expressed in many tissues and cell lines (1,2).

Application Details

Application Notes: Western blot at 2-4 ug/ml dilution. Recommended Positive Control: HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of 15 ug of total cell lysate from HeLa cells with anti-Caspase-10 at 4 ug/ml

Publications

Fernandes-Alnemri, Armstrong, Krebs et al.: "In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 15, pp. 7464-9, 1996 (PubMed).

Vincenz, Dixit: "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6578-83, 1997 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).