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Details for Product No. ABIN123306

Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 345-359), (AA 366-383) antibody

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Antigen
Synonyms APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, AI115399, AW493809, CASP-9, Caspase-9, Mch6, Apaf3, Casp9_v1, Ice-Lap6, Casp-9-CTD, CASP9
Epitope
»Alternatives AA 345-359, AA 366-383
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN123306
Quantity 0.2 mL
Price
445.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Mixture of synthetic peptides corresponding to amino acids 345-359 and 366-383 of rat caspase-9-CTD
Purification Protein G Chromatography
Alternative Name Caspase-9 / CASP-9-CTD
Background Caspase-9 and Apaf-1 bind to each other, which leads to Caspase-9 activation. Activated caspase-9 cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis inducers including TNFa, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in variety of human tissues. Recently, a novel isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. This protein called, Casp-9-CTD (where CTD is carboxyl-terminal divergent) is expressed in multiple tissues, with the relative highest statement observed in ovary and heart. The variant C terminus of Casp-9-CTD in rat is derived from an alternative exon of the rat Caspase-9 gene. Casp-9-CTD was found primarily in the cytoplasm and was not detected in the nucleus. Caspase-9-CTD proenzyme is not processed in the cells and lacks apoptotic activity. The Casp-9-CTD transfected cells are resistant to caspases induction by cytochrome c, suggesting that Casp-9-CTD acts as a dominant-negative variant.
Research Area Apoptosis/Necrosis
Application Notes Western blot (1-2 ug/ml, HeLa, mouse or rat heart whole cell lysate can be used as positive control and a 45-kDa band is detected). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Pan, ORourke, Dixit: "Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex." in: The Journal of biological chemistry, Vol. 273, Issue 10, pp. 5841-5, 1998 (PubMed).

Alternatives for antigen "Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)", type "Antibodies"
Hosts (297), (31), (1)
Reactivities (302), (102), (87), (20), (18), (6), (5), (3), (3), (3), (3), (3), (2)
Applications (276), (114), (72), (52), (50), (48), (47), (27), (9), (5), (4), (2), (1)
Conjugates (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (22), (21), (18), (17), (16), (12), (8), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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