Chemokine (C-C Motif) Receptor 1 (CCR1) antibody

Antigen: Chemokine (C-C Motif) Receptor 1 (CCR1)

Synonyms: CKR1, CD191, CKR-1, HM145, CMKBR1, MIP1aR, SCYAR1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN123307

Additional Information

Alternative name: CD191 / CCR1

UniProt: P32246

Immunogen: Synthetic peptide corresponding to amino acids 339-350 of human CCR1 protein AA Sequence:VSSTSPSTGEHEC

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Description: Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5, 6). Alternate Names: CD191.

Application Details

Application Notes: Western blotting: 1-3 ug/ml. Recommended Positive Control: A375 (a band of approx. size 42 kD should be detected). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.0 2% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chemokines, Receptors

Restrictions: For Research Use only

Images

Western blot analysis of 10 ug of total cell lysate from A357 cells with ABIN123307 at 2 ug/ml dilution.

Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and CCR1 (ABIN123307) with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 µl (blue histogram) and 10 µl (green histogram). Data kindly provided by Corey Parlet, University of Iowa.

Publications

Gao, Murphy: "Cloning and differential tissue-specific expression of three mouse beta chemokine receptor-like genes, including the gene for a functional macrophage inflammatory protein-1 alpha receptor." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17494-501, 1995 (PubMed).

Neote, DiGregorio, Mak et al.: "Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor." in: Cell, Vol. 72, Issue 3, pp. 415-25, 1993 (PubMed).

Gao, Sen, Kitaura et al.: "Identification of a mouse eosinophil receptor for the CC chemokine eotaxin." in: Biochemical and biophysical research communications, Vol. 223, Issue 3, pp. 679-84, 1996 (PubMed).

Gao, Wynn, Chang et al.: "Impaired host defense, hematopoiesis, granulomatous inflammation and type 1-type 2 cytokine balance in mice lacking CC chemokine receptor 1." in: The Journal of experimental medicine, Vol. 185, Issue 11, pp. 1959-68, 1997 (PubMed).

Broxmeyer, Cooper, Hangoc et al.: "Dominant myelopoietic effector functions mediated by chemokine receptor CCR1." in: The Journal of experimental medicine, Vol. 189, Issue 12, pp. 1987-92, 1999 (PubMed).