Chemokine (C-C Motif) Receptor 3 (CCR3) antibody

Antigen: Chemokine (C-C Motif) Receptor 3 (CCR3)

Synonyms: CKR3, CD193, CMKBR3, CC-CKR-3, MGC102841

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123309

Additional Information

Alternative name: CD193 / CCR3

UniProt: P51677

Immunogen: Synthetic peptide of human CCR3 protein

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR3 (eotaxin receptor), is expressed on eosinophils and certain T cell population respond to a variety of CC chemokines apart from eotaxin, including RANTES, monocyte chemotactic protein (MCP)-2, MCP-3, and MCP-4 (2,3). CCR3 facilitated infection by a more restricted subset of primary viruses, and binding of the CCR3 ligand, eotaxin, and inhibited infection by these isolates (4).

Specificity: This antibody is specific for CCR3.

Application Details

Application Notes: Western blot: 1 - 3 ug/ml. Recommended Positive Control: Daudi (a 45 kDa band should be observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chemokines, Receptors, CD Antigens

Restrictions: For Research Use only

Images

Western blot analysis of 10 ug of total cell lysate from Daudi cells with ABIN123309 at 2 ug/ml dilution.

Publications

Choe, Farzan, Sun et al.: "The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates." in: Cell, Vol. 85, Issue 7, pp. 1135-48, 1996 (PubMed).

Sallusto, Mackay, Lanzavecchia: "Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells." in: Science (New York, N.Y.), Vol. 277, Issue 5334, pp. 2005-7, 1997 (PubMed).