Chemokine (C-C Motif) Receptor 4 (CCR4) antibody

Antigen: Chemokine (C-C Motif) Receptor 4 (CCR4)

Synonyms: CKR4, K5-5, CD194, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN123310

Additional Information

Alternative name: CD194 / CCR4

Swiss-Prot: P51679

Immunogen: Synthetic peptide of human CCR4 protein. AA Sequence: IYSADTPSSSYTQSTC

Description: CCR4 is a chemokine receptor and is preferentially expressed on type 2 helper T (Th2-type) cells. In contrast to other chemokine receptors, the expression of CCR4 and CCR8 on Th2 cells is transiently increased following TCR and CD28 engagement. Moreover, activated Th1 cells up- regulate CCR4 expression and functional responsiveness to thymus- and activation-regulated chemokine. Alternate Names: CMKBR4, C-C CKR-4, CC-CKR-4, CCR-4, K5-5, C-C Chemokine Receptor 4, Chemokine (CC motif) Receptor 4.

Specificity: This antibody is specific for CCR4. Gen Bank no. ref|NP_005499.1|

Application Details

Application Notes: Western blot (1-2 ug/ml). Recommended Positive Control: Daudi (a band with an approx. size 42 kDa should be detected). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chemokines, Receptors

Restrictions: For Research Use only

Images

Western blot analysis of CCR4 in A) Daudi and B) NIH 3T3 cell lysate using ABIN123310 at 1 µg/ml.

Publications

Publications: Gao, Murphy: "Cloning and differential tissue-specific expression of three mouse beta chemokine receptor-like genes, including the gene for a functional macrophage inflammatory protein-1 alpha receptor." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17494-501, 1995 (PubMed).

Neote, DiGregorio, Mak et al.: "Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor." in: Cell, Vol. 72, Issue 3, pp. 415-25, 1993 (PubMed).

Murphy: "Chemokine receptors: structure, function and role in microbial pathogenesis." in: Cytokine & growth factor reviews, Vol. 7, Issue 1, pp. 47-64, 1997 (PubMed).

Broxmeyer, Cooper, Hangoc et al.: "Dominant myelopoietic effector functions mediated by chemokine receptor CCR1." in: The Journal of experimental medicine, Vol. 189, Issue 12, pp. 1987-92, 1999 (PubMed).