Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D)

Synonyms: DCR2, CD264, TRUNDD, TRAILR4, TNFRSF10D

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN123328

Additional Information

Alternative name: CD264 / TRAILR4

UniProt: Q9UBN6

Immunogen: Synthetic peptide corresponding to amino acids 249 to 263 of human DcR2 precursor (2)

Cross-Reactivity: Human

Description: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a decoy receptor (1). Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL/R4, or TRUNDD (2). DcR2 has an extracellular TRAIL-binding domain but lacks intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuated TRAIL-induced apoptosis. Alternate Names: TRAIL-R4, CD264.

Application Details

Application Notes: Western blot: 1:1000 to 1:2000 dilution. Recommended Positive Control: HeLa (a band of approximate molecular weight 35 kD will be detected). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of 50 ug of whole cell lysate from HeLa cells with anti-DcR2 at 1:1000 dilution

Immunohistochemical staining of HeLa cells using anti-DcR2 (ID) at 10 µg/ml.

Publications

Pan, ORourke, Chinnaiyan et al.: "The receptor for the cytotoxic ligand TRAIL." in: Science (New York, N.Y.), Vol. 276, Issue 5309, pp. 111-3, 1997 (PubMed).

Marsters, Sheridan, Pitti et al.: "A novel receptor for Apo2L/TRAIL contains a truncated death domain." in: Current biology : CB, Vol. 7, Issue 12, pp. 1003-6, 1998 (PubMed).

Mitsiades, Poulaki, Tseleni-Balafouta et al.: "Thyroid carcinoma cells are resistant to FAS-mediated apoptosis but sensitive to tumor necrosis factor-related apoptosis-inducing ligand." in: Cancer research, Vol. 60, Issue 15, pp. 4122-9, 2000 (PubMed).