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Details for Product No. ABIN123329

DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term), (AA 313-331) antibody

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Antigen
Synonyms DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA
Epitope
»Alternatives C-Term, AA 313-331
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN123329
Quantity 0.1 mg
Price
654.50 $   Plus shipping costs $45.00
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Availability Discontinued
Immunogen Peptide corresponding to amino acids 313-331 at the C-terminus of human DFF1
Purification Protein G affinity chromatography
Alternative Name DFFA / ICAD
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). A mouse homologue of human DFF was identified as a DNase inhibitor and designated ICAD (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4).
UniProt O00273
Research Area Apoptosis/Necrosis
Application Notes Immunoprecipiation. Western blot analysis (1:1000 -1:2000): A 45 kDa band should be observed. Positive control: HeLa cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody at 2-8°C. Shelf Life: one year from despatch.
Expiry Date 12 months
Ashihara, Takuya, Nishimura et al.: "[Immunohistochemical study of the human olfactory system]" in: Nippon Jibiinkoka Gakkai kaiho, Vol. 95, Issue 6, pp. 851-9, 1992 (PubMed).

Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Alternatives for antigen "DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA)", type "Antibodies"
Hosts (157), (24), (1)
Reactivities (124), (76), (22), (1), (1)
Applications (156), (59), (26), (24), (18), (14), (13), (10), (5), (4), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (40), (28), (12), (12), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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