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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody
|Synonyms||DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA, MGC143066, DKFZp469D0625|
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml) (Variants)|
|Price||594.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||DFFA / ICAD|
|Immunogen||Peptide corresponding to amino acids 313-331 at the C-terminus of human DFF1|
|Description||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). A mouse homologue of human DFF was identified as a DNase inhibitor and designated ICAD (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4).|
|Specificity||The antibody reacts with DFF.|
|Application Notes||Immunoprecipiation. Western blot analysis (1:1000 -1:2000): A 45 kDa band should be observed. Positive control: HeLa cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.02% sodium azide|
|Storage||Store the antibody at 2-8°C. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of 50 ug of total cell lysate from HeLa cells with anti-DFF antibody ABIN123329 at 1:2000 dilution. A 45 kDa band should be observed.|
Ashihara, Takuya, Nishimura et al.: "[Immunohistochemical study of the human olfactory system]" in: Nippon Jibiinkoka Gakkai kaiho, Vol. 95, Issue 6, pp. 851-9, 1992 (PubMed).
Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).
Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).
Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).
Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).
|Hosts||Rabbit (84), Mouse (12), Goat (1)|
|Reactivities||Human (64), Mouse (Murine) (38), Rat (Rattus) (7), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (91), ELISA (30), Immunohistochemistry (IHC) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (11), Immunofluorescence (IF) (8), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Epitopes||N-Term (21), C-Term (15), AA 2-21 (7), AA 312-331 (5), AA 313-331 (3), Transcript Variant 1 (3), AA 1-331 (2), Internal Region (2), N-Term,AA 2-21 (2), AA 31-2 (1), AA 312-331,C-Term (1), Asp224 (1), Cleaved-Asp224 (1)|