DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody

Antigen: DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA)

Synonyms: DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA, MGC143066, DKFZp469D0625

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN123329

Additional Information

Alternative name: DFFA / ICAD

UniProt: O00273

Immunogen: Peptide corresponding to amino acids 313-331 at the C-terminus of human DFF1

Cross-Reactivity: Human

Description: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). A mouse homologue of human DFF was identified as a DNase inhibitor and designated ICAD (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4).

Specificity: The antibody reacts with DFF.

Application Details

Application Notes: Immunoprecipiation. Western blot analysis (1:1000 -1:2000): A 45 kDa band should be observed. Positive control: HeLa cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2-8°C. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of 50 ug of total cell lysate from HeLa cells with anti-DFF antibody ABIN123329 at 1:2000 dilution. A 45 kDa band should be observed.

Publications

Ashihara, Takuya, Nishimura et al.: "[Immunohistochemical study of the human olfactory system]" in: Nippon Jibiinkoka Gakkai kaiho, Vol. 95, Issue 6, pp. 851-9, 1992 (PubMed).

Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).