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Deoxyribonuclease II, Lysosomal (DNASE2) (AA 347-360) antibody

Details for Product No. ABIN123330
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Antigen
Epitope
AA 347-360
(8), (5), (3), (1)
Reactivity
Human
(40), (15), (13), (11), (11), (11)
Host
Rabbit
(40), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(31), (13), (9), (9), (7), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
Catalog No. ABIN123330
654.50 $
Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide corresponding to amino acids 347 to 360 of human DNase precursor
Purification Protein G chromatography
Alternative Name Deoxyribonuclease-2-alpha
Background DNase II causes chromatin condensation and DNA fragmentation. The DNase II gene encodes for a proenzyme of approximate molecular mass of 40 kDa. The active enzyme consists of a heterodimer of 32 kDa and 12 kDa.
UniProt O00115
Application Notes Western blot: 2 - 4 ug/ml. Recommended Positive Control: human spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 347-360) antibody Western blot analysis of 50 ug of total cell lysate from HeLa cells with anti-DFF (CT) at 1:2000 dilution.
General Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN123330
654.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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