Deoxyribonuclease II, Lysosomal (DNASE2) antibody

Antigen: Deoxyribonuclease II, Lysosomal (DNASE2)

Synonyms: DNL, DNL2, DNASE2A, DNase, DNase 1, DNase-1, DNase1, dDNase II, Dmel\CG7780, CG7780, Dlad, AI526873, DnaseIIb, DLAD, UOX, Dnase2, DNASE2, MGC140819, DNASE2B, dnase2b, MGC140503, dnase2, MGC80103, LOC733868, fa92d01, MGC175261, wu:fa92d01, DKFZp469P2438

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN123330

Additional Information

Alternative name: Deoxyribonuclease-2-alpha

UniProt: O00115

Immunogen: Peptide corresponding to amino acids 347 to 360 of human DNase precursor

Cross-Reactivity: Human

Description: DNase II causes chromatin condensation and DNA fragmentation. The DNase II gene encodes for a proenzyme of approximate molecular mass of 40 kDa. The active enzyme consists of a heterodimer of 32 kDa and 12 kDa.

Synonyms: DNL, DNL2, DNASE2A, DNase, DNase 1, DNase-1, DNase1, dDNase II, Dmel\CG7780, CG7780, Dlad, AI526873, DnaseIIb, DLAD, UOX, Dnase2, DNASE2, MGC140819, DNASE2B, dnase2b, MGC140503, dnase2, MGC80103, LOC733868, fa92d01, MGC175261, wu:fa92d01, DKFZp469P2438

Application Details

Application Notes: Western blot: 2 - 4 ug/ml. Recommended Positive Control: human spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 50 ug of total cell lysate from HeLa cells with anti-DFF (CT) at 1:2000 dilution.

Publications

Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).