serine/threonine Kinase 17a (STK17A) antibody

Antigen: serine/threonine Kinase 17a (STK17A)

Synonyms: DAP6, EAP1, BING2, MGC126245, MGC126246, MGC150289, DAP-3, 4921514D13Rik, im:6905684, LOC100226911, DRAK1, STK17A, MGC140096

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123339

Additional Information

Alternative name: STK17A / DRAK1

UniProt: Q9UEE5

Immunogen: Peptide corresponding to amino acids 5 to 19 of human DRAK1 protein

Cross-Reactivity: Human

Description: Protein kinases play important roles in the signal transduction in response to a variety of external stimuli. Recently, several protein kinases have been identified that may also be involved in apoptotic process. Overexpression of a serine/threonine kinase, ZIP kinase can cause the morphological changes typical of apoptosis in NIH 3T3 cells (1). Sanjo et al, have identified two new protein kinases DRAK1 and DRAK2 (Death Receptor Associated Kinase 1 and 2) (1). Both DRAKs and ZIP kinase share significant homology at the amino acid level (1,2). The kinase domains of both DRAKs, ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-g. Overexpression of DRAKs in NIH 3T3 cells lead to apoptosis. These transfection experiments suggest that C- terminal domain of DRAKs are important for induction of apoptosis.

Application Details

Application Notes: Western blot: 2 - 4 ug/ml. Recommended positive control: HeLa. A ~66 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis for DRAK1 using ABIN123339 at 2 ug/ml dilution against 10 ug/lane of MOLT4 (lane 1), A431 (lane 2) and NIH-3T3 (lane 3) cell lysates.

Immunohistochemical staining of Molt4 cells using anti-DRAK1 (NT) at 2 µg/ml.

Publications

Sanjo, Kawai, Akira: "DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 29066-71, 1998 (PubMed).