Histone Deacetylase 4 (HDAC4) antibody

Antigen: Histone Deacetylase 4 (HDAC4)

Synonyms: HD4, HDACA, HA6116, HDAC-A, KIAA0288, Hdac4, mHDA1, AI426555, mKIAA0600, AI047285, 4932408F19Rik, wu:fa96d08, wu:fc56f08, zgc:152701, CG1770, dHDAC4, DHDAC4, DmelCG1770, dmHDA405, GC1770, HDAC, HDAC4a, dana_GLEANR_19966, DanaGF18710, GF18710, HDAC4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Chromatin Immunoprecipitation (ChiP)

Catalog no.: ABIN123352

Additional Information

Alternative name: HDAC4

UniProt: P56524

Immunogen: Synthetic peptide corresponding to amino acids 194-209 of human HDAC4

Cross-Reactivity: Human, Mouse (Murine)

Description: Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Specificity: This antibody is specific for Histone deacetylase 4.

Synonyms: HD4, HDACA, HA6116, HDAC-A, KIAA0288, Hdac4, mHDA1, AI426555, mKIAA0600, AI047285, 4932408F19Rik, wu:fa96d08, wu:fc56f08, zgc:152701, CG1770, dHDAC4, DHDAC4, DmelCG1770, dmHDA405, GC1770, HDAC, HDAC4a, dana_GLEANR_19966, DanaGF18710, GF18710, HDAC4

Application Details

Application Notes: Western blot: 0.5 - 2.0 ug/ml. ChIP:Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details. Immunoprecipitation. Recommended positive control: Jurkat or NIH 3T3. A 140 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % BSA and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cardiovascular, Hypertrophy, DNA/RNA, Transcription Factors, Wnt Signaling

Restrictions: For Research Use only

Images

Western blot analysis of HDAC4 in Jurkat cell lysate with ABIN123352.

Publications

Cress, Seto: "Histone deacetylases, transcriptional control, and cancer." in: Journal of cellular physiology, Vol. 184, Issue 1, pp. 1-16, 2000 (PubMed).

Meinke, Liberator: "Histone deacetylase: a target for antiproliferative and antiprotozoal agents." in: Current medicinal chemistry, Vol. 8, Issue 2, pp. 211-35, 2001 (PubMed).