Histone Deacetylase 5 (HDAC5) antibody

Antigen: Histone Deacetylase 5 (HDAC5)

Synonyms: HD5, NY-CO-9, FLJ90614, Hdac4, mHDA1, AI426555, mKIAA0600, Hd6, Sfc6, Hdac5, mHDA2, HDAC5, MGC134463, HDA5, HISTONE DEACETYLASE 5, MAF19.7, MAF19_7, DKFZp459O115

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123353

Additional Information

Alternative name: HDAC5

UniProt: Q9UQL6

Immunogen: A synthetic peptide corresponding to amino acids 572-587 of human HDAC5

Cross-Reactivity: Human, Mouse (Murine)

Description: Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Application Details

Application Notes: Immunoprecipitation. ChIP: Refer to Imbriano (2005) for details. Western blot: 2.0 ug/ml. A 120 kDa band is observed. Recommended positive control: 293, Jurkat, or NIH 3T3. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cardiovascular, Hypertrophy, Wnt, DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of HDAC5 in 293 (lane 1), Jurkat (lane 2) and NIH-3T3 (lane 3) cell lysate with ABIN123353.

Publications

Cress, Seto: "Histone deacetylases, transcriptional control, and cancer." in: Journal of cellular physiology, Vol. 184, Issue 1, pp. 1-16, 2000 (PubMed).

Meinke, Liberator: "Histone deacetylase: a target for antiproliferative and antiprotozoal agents." in: Current medicinal chemistry, Vol. 8, Issue 2, pp. 211-35, 2001 (PubMed).