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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody
|Synonyms||DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA, MGC143066, DKFZp469D0625|
Alternatives Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml) (Variants)|
|Price||594.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||DFFA / ICAD|
|Immunogen||Peptide corresponding to amino acids 312 to 331 of mouse ICAD (2)|
|Description||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase- activated DNase (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4). Alternate Names: DFF45.|
|Application Notes||Western blot: 1/500 - 1/1000. Recommended positive control: mouse lung. A 45 kDa band should be observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.02% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of 50 ug of mouse lung tissue lysate with anti-ICAD (CT) at 1:500 dilution.|
Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).
Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).
Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).
Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).
Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).
|Hosts||Rabbit (89), Mouse (12), Goat (1)|
|Reactivities||Human (68), Mouse (Murine) (40), Rat (Rattus) (8), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (96), ELISA (30), Immunohistochemistry (IHC) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunoprecipitation (IP) (12), Immunofluorescence (IF) (9), Immunocytochemistry (ICC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Fixed) (IHC (fx)) (3), Dot Blot (Dot) (1)|
|Epitopes||N-Term (16), C-Term (9), AA 2-21 (6), AA 312-331 (5), AA 313-331 (3), Transcript Variant 1 (3), AA 1-331 (2), Internal Region (2), N-Term,AA 2-21 (2), AA 31-2 (1), AA 312-331,C-Term (1), Asp224 (1), Cleaved-Asp224 (1)|