Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 175-188), (AA 526-540) antibody

Details for Product No. ABIN123361
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Antigen
Epitope
AA 175-188, AA 526-540
(15), (15), (12), (7), (7), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(100), (40), (35), (29), (12), (12)
Host
Rabbit
(88), (21), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB)
(78), (40), (29), (21), (18), (4), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN123361
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Immunogen Synthetic peptides corresponding to amino acids 175-188, 526-540, and 567-580 of humanIKKi/e proteinAA Sequence: GYNEEQIHKLDKVN / GTEEYLHPDMYERACRemarks: GenBank no. gb|AAI05925. 1|
Characteristics Synonyms: I kappa-B kinase epsilon, IKK-epsilon, IKK-E, IKKI, KIAA0151, Inducible I kappa-B kinase,IKK-I, Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Purification Protein G chromatography
Alternative Name IKBKE / IKKE
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitoryproteins that mask the nuclear localization signal of NF-kB thereby preventingtranslocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor orother cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinasecomplex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using asubtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPSincreases IKKi mRNA level in mouse macrophage cell lines. This protein has significantsequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wildtype IKKi in cells phosphorylates Ser32 and Ser36 of IkBa.
Gene ID 9641
UniProt Q14164
Application Notes Western blotting: 2 ug/ml. Recommended Positive Control: 293, Daudi, or Jurkat. An 80 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% gelatin and 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 175-188), (AA 526-540) antibody Western blot analysis of 30 ug of total cell lysate from Daudi cells with ABIN123361 at 2 ug/ml dilution.
General Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Hara, Ikeda, Shimada et al.: "[Surgical experience of 6 multiple primary lung cancers]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 3, pp. 183-7, 2008 (PubMed).

Validation Images
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