Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE)

Synonyms: IKKE, IKKI, IKK-i, KIAA0151, MGC125294, MGC125295, MGC125297, zgc:100844, IKBKE, MGC137373, MGC130907

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN123361

Additional Information

Characteristics: Synonyms: I kappa-B kinase epsilon, IKK-epsilon, IKK-E, IKKI, KIAA0151, Inducible I kappa-B kinase,IKK-I, Inhibitor of nuclear factor kappa-B kinase subunit epsilon

Alternative name: IKBKE / IKKE

Gene ID: 9641

Swiss-Prot: Q14164

Immunogen: Synthetic peptides corresponding to amino acids 175-188, 526-540, and 567-580 of humanIKKi/e proteinAA Sequence: GYNEEQIHKLDKVN / GTEEYLHPDMYERACRemarks: GenBank no. gb|AAI05925. 1|

Description: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitoryproteins that mask the nuclear localization signal of NF-kB thereby preventingtranslocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor orother cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinasecomplex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using asubtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPSincreases IKKi mRNA level in mouse macrophage cell lines. This protein has significantsequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wildtype IKKi in cells phosphorylates Ser32 and Ser36 of IkBa.

Specificity: This antibody is specific for IKKe/IKKi. Species: Human. Others not tested.

Application Details

Application Notes: Western blotting: 2 ug/ml. Recommended Positive Control: 293, Daudi, or Jurkat. An 80 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 30 ug of total cell lysate from Daudi cells with ABIN123361 at 2 ug/ml dilution.

Publications

Publications: Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Ruegnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Hara, Ikeda, Shimada et al.: "[Surgical experience of 6 multiple primary lung cancers]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 3, pp. 183-7, 2008 (PubMed).