Suppressor of Cytokine Signaling 5 (SOCS5) antibody

Antigen: Suppressor of Cytokine Signaling 5 (SOCS5)

Synonyms: CIS6, CISH6, Cish5, SOCS-5, KIAA0671, mKIAA0671, 1810018L08Rik, SOCS5, RGD1564914, MGC137606, cis6, cish5, cish6, socs-5, Socs-5, GB18949, socs5, zgc:153506

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN123365

Additional Information

Alternative name: SOCS5 / CIS6

UniProt: O75159

Immunogen: Synthetic peptide containing amino acid residues of human SOCS5 protein

Cross-Reactivity: Human, Mouse (Murine)

Description: The suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS) inhibit cytokine production by a classical negative feedback mechanism. These proteins are upregulated in response to cytokine stimulation followed by inhibition of cytokine-inducing signal pathway. Role of each member of the family has been determined by gene knockout experiments (1). SOCS5 protein is preferentially expressed in committed T-helper (Th1) cells. The action of SOCS5 is probably mediated by its interaction with the cytoplasmic domain of IL-4 receptor. During Th1 differentiation, there is a reduction in the association of Janus kinase 1 with the IL-4 receptor (IL-4R), which is correlated with the expression of SOCS5.pressor of cytokine signaling-5 (SOCS5). It has been also shown that in the overexpression of SOCS5 in transgenic mice leads to impaired Th2 development, suggesting that SOCS5 may plan an important role in the Th1 differentiation by regulating a balance between Th1 and Th2 (2).

Application Details

Application Notes: Western blot: 1:500 dilution. Use human or mouse spleen lysate for positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide and 0.1 mg/ml BSA

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis for SOCS5 using ABIN123365 at 1:500 dilution against 15 ug/lane of mouse spleen (lane A) and human spleen (lane B) lysates.

Publications

Krebs, Hilton: "SOCS proteins: negative regulators of cytokine signaling." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 5, pp. 378-87, 2001 (PubMed).

Seki, Hayashi, Matsumoto et al.: "Expression of the suppressor of cytokine signaling-5 (SOCS5) negatively regulates IL-4-dependent STAT6 activation and Th2 differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 13003-8, 2002 (PubMed).