Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A) antibody

Antigen: Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A)

Synonyms: LANP, MAPM, PP32, PHAP1, PHAPI, I1PP2A, C15orf1, MGC119787, MGC150373, pp32, Anp32, W91701, MGC105504, anon-EST:fe3A2, Anp32a, CG5784, CT18148, CT42180, DmelCG5784, zgc:55516, wu:fj36e08, ANP32A, HPPCn, ANP32D

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Chimpanzee, Human, Rhesus Monkey

Application: Western Blotting (WB)

Catalog no.: ABIN123369

Additional Information

Characteristics: Synonyms: C15orf1, LANP, MAPM, PHAP1, Mapmodulin, Acidic leucine-rich nuclear phosphoprotein 32family member A, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Acidicnuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, PutativeHLA-DR-associated protein I

Alternative name: ANP32A

Gene ID: 8125

Swiss-Prot: P39687

Immunogen: Synthetic peptides of amino acids 87-103 and 231-246 of human INHAT-2AA Sequence: CPNLTHLNLSGNKIKDL / EERGQKRKREPEDEGE

Description: INHAT (inhibitor of acetyltransferases) complex binds to histones and masks them frombeing substrate for acetyltransferase. Endogenous INHAT subunits, which include theSet/TAF-Ibeta oncoprotein, associate with chromatin in vivo and can block coactivatormediated transcription when transfected in cells.

Specificity: This antibody is spedific for INHAT-2/pp32. Species: Human, Rhesus Monkey, Chimpanzee, Cow. Predicted to react with Mouse, Rat,Chicken. Others not tested.

Synonyms: LANP, MAPM, PP32, PHAP1, PHAPI, I1PP2A, C15orf1, MGC119787, MGC150373, pp32, Anp32, W91701, MGC105504, anon-EST:fe3A2, Anp32a, CG5784, CT18148, CT42180, DmelCG5784, zgc:55516, wu:fj36e08, ANP32A, HPPCn, ANP32D

Application Details

Application Notes: Western blot: 1/500 - 1/1000. Recommended positive control: HeLa. In HeLa, a 31 kDa band is observed. Otherapplications not tested. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.02% sodium azide and 0.1 mg/ml BSA

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot detection of INHAT-2 using ABIN123369 at a 1:1000 dilution against 10 ug of HeLa cell lysate.

Publications

Publications: Seo, Macfarlan, McNamara et al.: "Regulation of histone acetylation and transcription by nuclear protein pp32, a subunit of the INHAT complex." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 14005-10, 2002 (PubMed).