Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) antibody

Antigen: Interleukin-1 Receptor-Associated Kinase 4 (IRAK4)

Synonyms: IPD1, REN64, NY-REN-64, IRAK4, IRAK-4, 8430405M07Rik, 9330209D03Rik, MGC55553, zgc:55553, MGC137816, ren64, ny-ren-64, irak4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN123372

Additional Information

Alternative name: IRAK4

UniProt: Q9NWZ3

Immunogen: Mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members (2). The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified (3). IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges (4, 5).

Application Details

Application Notes: Western blot (1:500-1:1000, Positive control: NIH 3T3). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Immunology, Innate Immunity, Atherosclerosis, Cytokines, Receptors, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using ABIN123372 at 1:500 dilution.

Publications

Cao, Xiong, Takeuchi et al.: "TRAF6 is a signal transducer for interleukin-1." in: Nature, Vol. 383, Issue 6599, pp. 443-6, 1996 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Lai, Chen, Fen et al.: "[Genetic polymorphism of fifteen short tandem repeat loci in Chinese Drungs]" in: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, Vol. 19, Issue 6, pp. 502-4, 2002 (PubMed).

Shirai, Koizumi, Ogawa et al.: "[Anti-signal recognition particle (SRP) antibody positive polymyositis refractory to corticosteroid treatment]" in: Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine, Vol. 96, Issue 11, pp. 2522-4, 2007 (PubMed).